Working as a member of Team X, Zero helped Stryker find the metallic compound known as adamantium. He, along with Sabretooth, were the only ones from Team X that stayed faithful to Stryker when the team disbanded.
After Wolverine breaks out of the Weapon X facility, Stryker orders Zero to pursue him - eventually tracking him to a barn belonging to an elderly couple. Zero shoots the couple and blows up the barn in an attempt to kill Wolverine. Wolverine, however, escapes and is soon pursued by a Zero in a helicoptor.
Wolverine eventually brings the helicoptor down. Severely injured and trapped inside the helicoptor, Zero mocks Wolverine about how "innocent people die around him". As Wolverine walks away, he ignites a trail of fuel with his claws, causing the helicopter to explode and killing Zero.